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Santosh B. Dighe ¹, B. S. Kuchekar ², Sagar B. Wankhede ³

Affiliations
1 Department of Pharmacology, Pravara Rural College of Pharmacy, Pravranagar, Maharashtra, 413736, IN
2 Dept. of Pharmaceutical Chemistry, MAEER, Maharashtra Institute of Pharmacy, Pune Maharashtra, IN
3 Department of Pharmaceutical Chemistry, Pad. Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pune, Maharashtra, IN

Abstract

The present study aimed at the in-vitro comparative study of anthelmintic activity of petroleum ether, ethyl acetate and methanol extracts of whole plant of Oxalis corniculata Linn. using Eisenia foetida at three different concentrations (100, 200, and 400 mg/ml) respectively. The study involved the determination of time of paralysis (P) and time of death (D) of the worms. At the concentration of 400 mg/ml all the extracts showed very significant activities as compared to the standard drug levamisole (0.55 mg/ml). Each extract with the dose 100, 200, and 400 mg/ml, produced dose-dependent paralysis ranging from loss of motility to loss of response to external stimuli, which gradually progressed to death. In case of methanol extract which was found to be most potent, the time of paralysis and death time was observed as 11.33 and 41.33 min, respectively. In conclusion, the use of O. corniculata as possible anthelmintic is explored and further studies are suggested to isolate the active principles responsible for the activity.

Keywords

Anthelmintic, Eisenia foetida, Oxalis corniculata Linn.

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K. D. Ingale ¹, A. L. Barhate ¹, A. N. Kale ¹, C. D. Bobade ¹, V. P. Choudhari ¹, B. S. Kuchekar ¹

Affiliations
1 MAEER's Maharashtra Institute of Pharmacy, S.NO.124, MIT Campus, Ex-Serviceman Colony, Paud Road, Kothrud, Pune - 411 038, IN

Abstract

Two methods for simultaneous determination of Emtricitabine and Tenofovir Disoproxil Fumarate by spectroscopy have been developed. First method is Simultaneous equation method and second method is Absorbance ratio Method. From a solvent effect studies and the spectral behaviours of Emtricitabine and Tenofovir Disoproxil Fumarate, methanol was selected as solvent. Emtricitabine shows maximum absorbance at 281 nm and Tenofovir Disoproxil Fumarate shows maximum absorbance at 259 nm. Linearity in concentration range of 6-48 μg/mL and 4-32 μg/ mL was shown for Tenofovir Disoproxil Fumarate and Emtricitabine, respectively. In the first method, the concentration of the drugs were determined by using simultaneous equation and in second method the concentration of drugs were determined by using ratio of absorbances at isoabsorptive point and at the ëmax of one of the drug. The results of analysis were validated statistically and by recovery studies. Tablet containing both drugs was assayed using the methods developed, showing a good accuracy and precision.

Keywords

Emtricitabine, Tenofovir Disoproxil Fumarate, Simultaneous Equation Method, Absorbance Ratio Method, Pharmaceutical Formulation.

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A. R. Chabukswar ¹, A. K. Kolsure ¹, B. S. Kuchekar ¹, S. C. Jagdale ¹

Affiliations
1 MAEER'S Maharashtra Institute of Pharmacy, S. No. 124, MIT Campus, Paud Road, Kothrud, Pune - 411 038, IN

Abstract

A simple, economical, precise and accurate method for simultaneous determination of valsartan (VAL) and amlodipine besylate (AML) in combined capsule dosage form has been developed. The method is based on ratio spectra derivative spectrophotometry. The amplitudes 229.48 nm and 299.10 nm in the first derivative of the ratio spectra were selected to determine valsartan (VAL) and amlodipine besylate (AML) respectively in combined formulation. Beer's law is obeyed in the concentration range of 8-40 μg mL^-1 for VAL and 0.5-2.5 μg mL^-1 for AML for the methods. The % assay for commercial formulation was found to be 98.56% ± 0.27 for VAL and 98.91% ± 0.51 for AML by the proposed methods. The methods were validated with respect to linearity, precision and accuracy. Recovery by both the methods was found in the range of 98.89 - 99.60 % for VAL and 98.58 - 99.63 % for AML.

Keywords

Valsartan and Amlodipine besylate, Ratio Spectra Derivative Spectrophotometry, Capsule Dosage Form.

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P. B. Aswar ¹, S. S. Khadabadi ², B. S. Kuchekar ³, R. Dhage ², S. P. Deshmukh ²

Affiliations
1 Government College of Pharmacy, Kathora Naka, Amravati- 444 604 (M.S.), IN
2 Government College of Pharmacy, Amravati 444604(M.S.), IN
3 MIT College of Pharmacy, Kothrud, Pune, IN

Abstract

Enicostema axillare is well known for its anthelmintic and bacterial activity. In present study dried leaves and ischolar_mains of Enicostema axillare were subjected to exhaustic sequential extraction with four solvent i.e.-hexane, chloroform, methanol and distilled water respectively. All four extracts were studied for in vitro licicidal activity using goat-lice Damalinia caprae (Trichodectidae) as experimental organism. Only n-hexane and chloroform fractions showed statistically significant licicidal activity (p value <0.001, n = 5). Decrease in mean time required to kill the lice was observed at concentration 1% w/w and 10% w/w when compared to 1% lindane solution.

Keywords

Enicostema axillare Leaves and Roots, Licicidal Activity.

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A. S. Bhadoriya ¹, S. S. Padekar ¹, V. D. Patil ¹, S. N. Shinde ¹, V. P. Choudhari ¹, B. S. Kuchekar ¹

Affiliations
1 Deparment of Pharmaceutical Analysis and Quality Assurance, MAEER’s Maharashtra Institute of Pharmacy, Kothrud, Pune-411038, IN

Abstract

A simple, economical, precise and accurate ratio spectra derivative spectrophotometric method for Aspirin (ASP), Atenolol (ATN), and Amlodipine besylate (AML) ternary combination on laboratory prepared tablet formulation has been developed. The method is based on obtaining first derivative of ratio spectra and measuring amplitude at selected wavelength. The derivative amplitudes at 312.5 nm, 315.5 nm and 347.0 nm were selected for measuring the response of Aspirin (ASP), Atenolol (ATN), and Amlodipine besylate (AML), respectively. Beer’s law is obeyed in the concentration range of 20-100 μg ml^-1 for ASP and 10-50 μg ml^-1 for ATN and 1-5 μg ml^-1 AML. The % assay was found to be in the range 98.0-101.0% for ASP and 98.91-101.72% for ATN and 98.71-101.2% for AML by the proposed method. The method was validated with respect to linearity, precision and accuracy. Recovery was found in the range of 98.72-100.64% for ASP and 98.45-101.21% for ATN and 98.32-101.12% for AML.

Keywords

Aspirin, Amlodipine, Atenolol, Ratio Spectra, First Derivative.

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A. R. Chabukswar ¹, S. N. Shinde ¹, B. S. Kuchekar ¹, S. C. Jagdale ¹, P. D. Lokhande ², V. P. Choudhari ¹, K. D. Ingale ¹

Affiliations
1 MAEER’S Maharashtra Institute of Pharmacy, S. No. 124, MIT Campus, Paud Road, Kothrud, Pune-411038, IN
2 Department of Chemistry, University of Pune, Ganeshkhind Road, Pune-411007, IN

Abstract

Simultaneous determination of Irbesartan (IRBE) and Hydrochlorothiazide (HTZ) in combined tablet dosage form has been developed by ratio spectra derivative and absorption correction spectrophotometry method. The amplitudes at 252.6 nm and 230 nm in the first derivative of the ratio spectra method were selected to determine IRBE and HTZ respectively and the second method was based on the absorption correction method in which IRBE and HTZ exhibit λ_max at 244 nm and 275.02 nm, respectively in 0.1 N HCL. HTZ has some interference due to IRBE at 275.02 nm, while HTZ do not show any absorption at 244 nm. The % assay for commercial formulations was found to be in the range 98.29-99.80 for IRBE and 98.91-100.10% for HTZ by the proposed methods, for both the formulations. Recovery was found in the range of 98.11-100.08% for IRBE and 98.54-99.29% for HTZ by ratio derivative method and 98.59-99.56% for IRBE and 99.33-100.12% for HTZ by absorbance correction method for both the Formulations. The methods were validated and found to be linear, precise and accurate, as per ICH guidelines. Absorbance correction method was applied for dissolution studies and drug release pattern was evaluated.

Keywords

Irbesartan and Hydrochlorothiazide, Ratio Derivative, Absorption Correction, Dissolution.

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V. H. Potdar ¹, B. S. Kuchekar ², S. S. Patil ¹

Affiliations
1 Tatyasaheb Kore College of Pharmacy, Warananagar-416113, Maharashtra, IN
2 MAEER’S Maharashtra Institute of Pharmacy, Kothrud, Pune, Maharashtra, IN

Abstract

The objective of this work was to develop a simple, accurate, rapid and sensitive method for the estimation of ambroxol hydrochloride in presence of cetirizine hydrochloride in tablets. The estimation is based on the reduction of ferric from ferric nitrate to ferrous ions by the drug (ambroxol hydrochloride), which further in presence of 3-Methyl-2-Benzothiazolinone Hydrazone Hydrochloride (MBTH), to produce red colored complex, measured at 568 nm. The chromogen obeyed linearity over 6-54 μg/ml (r=0.9988) with percent relative standard deviation (% RSD) of 0.16, showing that method has good accuracy and precision.

Keywords

Ambroxol Hydrochloride, Cetirizine Hydrochloride, MBTH, Ferric Nitrate.

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